Regulatory

Part:BBa_K4708001

Designed by: Olivia Gibson   Group: iGEM23_BostonU-HW   (2023-10-05)


pLac/RhlI

Hybrid promoter that can drive the expression of RhlI and fluorescence proteins. The promoter is constitutively on unless LacI is present and the cell is induced with IPTG or a quorum signaling molecule. The cell can act as a sender in a quorum signaling pathway, since it promotes the expression of RhlI, producing C4HSL. The cell with this promoter can also act as a receiver cell in a quorum signaling pathway if LacI is targeted and induced by the signaling molecule (repressing the promoter's activity).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None